![]() ![]() This method does not offer any information on the size of the target protein.During the dot blotting, the electrophoresis of the protein samples not performed instead they are directly applied on a membrane in a single spot, and the blotting method is conducted.Dot Blot is a simplified technique of western blotting, which is mainly used for the detection of proteins.There are different blotting techniques such as Southern blotting, western blotting, and Northern blotting, etc. It can also be used in the identification of the causative agent of any infectious disease.Dot blot is a blotting technique used for the detection of proteins.It is used in DNA fingerprinting and restriction fragment length polymorphism. ![]() Used for paternity testing along with the identification of criminals and victims.The technique can be used for DNA analysis to detect point mutations and other structural rearrangements in the DNA sequences.It can be used in diagnostic study of genetic diseases.It is applied in the field of gene mapping and evolution.It is used to detect and determine the size of specific DNA fragment in the given mixture of genomes.If a fluorescent or a chromogenic dye is used, the hybridized regions can be visualized on X-ray film or as colored spots on the membrane.The hybridized fragments on the membrane can be detected by autoradiography.Detection and visualization by autoradiography: The base pairing between the probe and ssDNA on the membrane forms a hybrid DNA molecule by the process called hybridization.ħ.The probe can be labeled with any radioactive substance or dyes with fluorescence or chromogenic property.The membrane is then incubated with a labeled probe which contains the complementary sequences to the gene of interest on the membrane.The size of the DNA fragments can be determined by comparing their relative size with the DNA bands of known lengths.The position of the band containing these hybridized fragments is determined by autoradiography or any other method depending on the types of probes used.The probe hybridizes with the immobilized ssDNA on the membrane having the gene of interest. ![]()
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